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Merck
CN

ST

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92040221, Porcine testis, Not specified

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About This Item

UNSPSC Code:
41106514
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biological source

Porcine testis

packaging

tube of 5 μg 92040221-DNA-5UG
pkg of vial of cells 92040221-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Biochem/physiol Actions

Porcine testis, foetal
The cell line was established in 1960 from the trypsinised testis of a normal 80-90 day old porcine foetus. The cells support the growth of various porcine parvo- and entero-viruses, and also pseudorabies virus. Also useful in the propagation and isolation of transmissible gastro-enteritis of swine.

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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