RENE 1

Derived from the endometrium tissue from the uterus of a 21-28 day old Sprague-Dawley rat. Cells carry vimentin, connexin 26 and connexin 43 markers. The transforming agent is adenovirus E1a protein.

Rat Sprague-Dawley, primary endometrial, adenovirus transformed

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time 48 hours.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.