biological source
rat endometrium
packaging
tube of 5 μg 92092516-DNA-5UG, pkg of vial of cells 92092516-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Cuboidal
products
Not specified
receptors
Glucocorticoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat, transformed with a ts mutant of SV40 T antigen (tsA58). Cells express vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43.
Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed
Preparation Note
DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 34°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.