Hepa 1-6

Hepa 1-6 has been used to study the biodistribution of polymer hydrogel capsules for the delivery of therapeutics. It has also been used to evaluate the potential of interferon regulatory factor-1 (IRF-1) for cytokine gene therapy of hepatocellular carcinoma (HCC).

Derived from BW7756 tumour in a C57L mouse. Secretes several liver products including albumin, α-fetoprotein, α 1-antitrypin and amylase.

Mouse hepatoma

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.