biological source
human colon
packaging
tube of 5 μg 93052620-DNA-5UG
pkg of vial of cells 93052620-1VL
growth mode
Semi-adherent
karyotype
Not specified
morphology
Predominantly round, refractile
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human colon carcinoma
Cell Line Description
COLO 206F, COLO 201 (ECACC Catalogue 87091201) and COLO 205 (ECACC Catalogue 87061208) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). The cell line can be used for immunological studies in cytotoxicity assays and immunoglobin characterisation. Please note this cell line was mycoplasma eradicated at ECACC.
Culture Medium
RPM11640, 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Subculture Routine
Maintain cultures between 3-9x105 cells/ml 5% CO2, 37°C. Any attached cells can be dislodged with trypsin/EDTA.
Other Notes
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