biological source
human colon
packaging
tube of 5 μg 93052620-DNA-5UG, pkg of vial of cells 93052620-1VL
growth mode
Semi-adherent
karyotype
Not specified
morphology
Predominantly round, refractile
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
COLO 206F, COLO 201 (ECACC Catalogue 87091201) and COLO 205 (ECACC Catalogue 87061208) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). The cell line can be used for immunological studies in cytotoxicity assays and immunoglobin characterisation. Please note this cell line was mycoplasma eradicated at ECACC.
Human colon carcinoma
Preparation Note
Maintain cultures between 3-9x105 cells/ml 5% CO2, 37°C. Any attached cells can be dislodged with trypsin/EDTA.
RPM11640, 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.