biological source
Mouse x Rat Hybridoma
packaging
tube of 5 μg 93091302-DNA-5UG, pkg of vial of cells 93091302-1VL
ImmunoDon
Fischer Rat spleen
Myeloma
Sp2/0
Reactivity
Anti-VLA-4 integrin-like cell adhesion molecule, murine
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Immunogen
P815 murine mastocytoma cells
Application
The isotype of the monoclonal antibody produced by this cell line is rat IgG2b.
Biochem/physiol Actions
Mouse Sp2/0 x Rat Fischer spleen
The antibody is directed against the α-4 chain of the murine VLA-4 integrin-like cell adhesion molecule which is present on blood cells. The antibody will react with human integrins.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM glutamine + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.