biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 93092704-DNA-5UG, pkg of vial of cells 93092704-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0 Ag-14
Reactivity
Anti-λ-Light chain, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
Purified human immunoglobulin myeloma protein covalently coupled to polyaminostyrene beads
Application
Classification of human imunoglobulin
Biochem/physiol Actions
Mouse Sp2/0 Ag-14 x Mouse BALB/c spleen
The antibody is directed against human kappa light chains.
Preparation Note
DMEM + 2mM glutamine + 10mM HEPES + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.