biological source
Porcine intestine
packaging
tube of 5 μg 93100622-DNA-5UG, pkg of vial of cells 93100622-1VL
growth mode
Adherent
karyotype
Heteroploid
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Studies of epithelial cell differentiation.
Biochem/physiol Actions
Boar Miniature male ileum, SV40 transformed
Epitheloid cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo. Electron microscopy analysis shows evidence of microvilli and desmosomes connecting cells. The cells were found to co-express cytokeratin 18 specific of simple luminal epithelial cells and vimentin associated with a mesenchymal origin. Susceptible to Transmissible Gastro-enteritis (TG) virus.
Preparation Note
DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.