biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 93113021-DNA-5UG, pkg of vial of cells 93113021-1VL
ImmunoDon
AO rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-MHC class II, ovine
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−196°C
Immunogen
Sheep afferent lymph cells
Biochem/physiol Actions
Cross reacts with human, murine and bovine MHC class II molecules; reacts with β chain of all MHC class II molecules expressed in sheep by immunoblot and cell surface staining. Inhibits proliferation.
Rat Y3-Ag1.2.3 x Rat AO spleen
Preparation Note
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C. Saturation density is reached at 3-9x100,000 cells/ml.
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.