biological source
rat liver
packaging
tube of 5 μg 93120108-DNA-5UG
pkg of vial of cells 93120108-1VL
growth mode
Adherent
karyotype
2n = 66, hypertriploid
morphology
Epithelial
products
Not specified
receptors
Dexamethasone phosphate; hydrocortisone
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and enzymatic studies
Biochem/physiol Actions
Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.
Rat hepatoma
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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