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Merck
CN

C1300 CLONE NA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93120817, mouse nerve, Neuronal-like

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About This Item

UNSPSC Code:
41106514
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biological source

mouse nerve

packaging

tube of 5 μg 93120817-DNA-5UG
pkg of vial of cells 93120817-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Neuronal-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Biochem/physiol Actions

CLONE NA is a subclone of Neuro-2a, a clonal line of C1300 mouse neuroblastoma cells. The cells were selected for spontaneous mutations deficient in HGPRT in the presence of 8-azaguanine. This cell line has been mycoplasma eradicated at ECACC.
Mouse neuroblastoma

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cultures may not form a complete monolayer before subculture is required.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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