QT 6

Virus studies

Derived from methylcholanthrene-induced fibrosarcoma of Japanese quail. The cells are susceptible to several viruses including sub group A avian sarcoma, avian encephalomyelitis, Marek′s disease herpes virus and rabies virus.

Quail Japanese fibrosarcoma

Ham′s F10 + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C; subculture at about 75% confluency; cells can be dislodged by tapping flask against the palm of hand; use of 0.5% polyvinylpyrrol

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.