biological source
rat brain
packaging
tube of 5 μg 94020810-DNA-5UG, pkg of vial of cells 94020810-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Gonadatrophs
receptors
Gonadotrophin-releasing hormone receptors
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.
Biochem/physiol Actions
RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSβ, LHβ and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.
Rat pituitary adenoma
Preparation Note
DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF.
Split sub-confluent cultures (70-80%) 1:6 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 10% CO2; 34°C. Saturation density is reached at 1x 100,000 cells/cm2. Cells may attach lightly and so handling should be kept to a minimum. Care shoul
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.