EoL-1 cell
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94042252, human blood, Lymphoblast
Synonym(s):
AML-EOL-1 Cells, EoL1 Cells, Eosinophilic Leukemia Cell Line
biological source
human blood
packaging
tube of 5 μg 94042252-DNA-5UG
pkg of vial of cells 94042252-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
EoL-1 cell line has been used to study the role of AGS67E, an antibody-drug conjugate that targets cluster of differentiation-37 (CD37) for the treatment of B/T-cell malignancies. It has also been used to study the role of anti-vascular cell adhesion molecule-1 (VCAM-1) monoclonal antibody in inhibiting asthma in a murine asthma model.
Biochem/physiol Actions
Differentiated by N-butylate treatment.
Human eosinophilic leukaemia
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 12,13
D16S539: 8,10
D5S818: 11
D7S820: 10,13
THO1: 9.3
TPOX: 8
vWA: 16,17
CSF1PO: 7,10
D13S317: 12,13
D16S539: 8,10
D5S818: 11
D7S820: 10,13
THO1: 9.3
TPOX: 8
vWA: 16,17
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Eol-1 cells grow very slowly and may take up to 5 days to reach a suitable level to subculture. It is best that these cells are left to grow and not agitated more than necessary.The cells actually grow as large clusters. They are beginning to reach confluence when the cells in the centre of each cluster start to look unhealthy. The change in media colour should also be an indication. Eol-1 cells prefer slightly acidic conditions and so may resuscitate better from frozen if started up in 15% foetal bovine serum.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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