biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 94050507-DNA-5UG, pkg of vial of cells 94050507-1VL
ImmunoDon
Mouse spleen
Myeloma
S194/5.XXO.BU.1
Reactivity
Anti-Haematopoietic Cell, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG
shipped in
dry ice
storage temp.
−196°C
Immunogen
K-562 cells
Biochem/physiol Actions
Mouse S194/5.XXO.BU.1 x Mouse spleen cells
The antibody is directed against an acidic glycoprotein (dimer of 2x110kDa monomers joined by disulphide bonds) present on immature and proliferating human haematopoietic cells but not circulating peripheral blood cells, or other non haematopoietic cells.
Preparation Note
DMEM + 2mM glutamine + 0.02mM 2-mercaptoethanol (2ME) + 10% Horse Serum.
Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.