biological source
Rat x Mouse Hybridoma
packaging
tube of 5 μg 94090613-DNA-5UG, pkg of vial of cells 94090613-1VL
ImmunoDon
Rat Lou/MN spleen cells
Myeloma
Sp2/0
Reactivity
Anti-Pgp-1, murine homologue of CD44
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Immunogen
BUS 2 cells
Application
Determination of ligand specifities and function of Pgp-1/CD44 molecules.
The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.
Biochem/physiol Actions
Rat Lou/MN spleen x Mouse Sp2/0
The hybridoma KM703 was produced by the fusion of Sp2/0 mouse myeloma cells with spleen cells from a Lou/MN rat immunised with the bone marrow derived stromal cell clone, BMS2. The monoclonal antibody recognises an epitope on Pgp-1, the murine homologue of CD44. It prevents the growth of lymphoid or myeloid cells in long term bone marrow cultures.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.