Psi-CRE

Ecotropic helper-free recombinant retroviral packaging cell line. Valuable for `in vivo′ gene transfer studies aimed at cell lineage analysis and development of human gene replacement therapy.

From a set of packaging cell lines useful for generating helper-free recombinant retroviruses with ectotropic host ranges. Two mutant Moloney murine leukaemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions were sequentially introduced into NIH 3T3 cells by co-transformation. Each genome contained a deletion of the Psi sequence necessary for the efficient encapsidation of retroviral genomes into virus particles and additional alterations at the 3′end of the provirus. Psi-CRE can be used to isolate clones that stably produce high titres (1000,000 cfu/ml) of recombinant retroviruses with ectotropic host ranges, viral producers derived from Psi-CRE do not transfer the packaging function or yield helper virus.

Mouse NIH 3T3 transformed with Moloney murine leukaemia mutants

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (NB. The results obtained by the depositor were gained using 10% Calf Serum (CS)).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5-10% CO₂, 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.