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About This Item
UNSPSC Code:
41106514
Biological source:
human nerve (bone marrow metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
46
Morphology:
Polygonal
biological source
human nerve (bone marrow metastasis)
packaging
tube of 5 μg 94092302-DNA-5UG, pkg of vial of cells 94092302-1VL
growth mode
Adherent
karyotype
46
morphology
Polygonal
products
Not specified
receptors
Vimentin
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
Neuroblastoma differentiation studies
Biochem/physiol Actions
Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.
Human neuroblastoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Regulatory Information
常规特殊物品
低风险生物材料
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