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Merck
CN

SK-N-AS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94092302, human nerve (bone marrow metastasis), Polygonal

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About This Item

UNSPSC Code:
41106514
Biological source:
human nerve (bone marrow metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
46
Morphology:
Polygonal
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biological source

human nerve (bone marrow metastasis)

packaging

tube of 5 μg 94092302-DNA-5UG, pkg of vial of cells 94092302-1VL

growth mode

Adherent

karyotype

46

morphology

Polygonal

products

Not specified

receptors

Vimentin

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Application

Neuroblastoma differentiation studies

Biochem/physiol Actions

Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.
Human neuroblastoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17

Preparation Note

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.


Regulatory Information

常规特殊物品
低风险生物材料

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