HEL 12469
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Synonym(s):
12469, HEL, HEL-12469, HEL12469, HEL1249
biological source
human lung (fetal)
packaging
tube of 5 μg 94101201-DNA-5UG
pkg of vial of cells 94101201-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Biochem/physiol Actions
Fibroblasts fuse when infected with human CMV at a high input multiplicity.
Human embryonic lung fibroblast
Not specified
Preparation Note
EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4, i.e., seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
Regulatory Information
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