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About This Item
UNSPSC Code:
41106514
Biological source:
rat prostate (ventral)
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial-like
biological source
rat prostate (ventral)
packaging
tube of 5 μg 94101450-DNA-5UG, pkg of vial of cells 94101450-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
AT-2.1 was developed via in vivo passages of the Dunning R-3327 rat prostatic cancers. A series of other cell lines were produced and they hold in common with Dunning R-3327 and AT-2.1 a structural change in chromosome 4 involving a translocation.
Rat prostatic cancer
Preparation Note
RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells/ml. Cells can be easily dislodged from the monolayer mechanically, however 0.25% trypsin or trypsin/EDTA can be used; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
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