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P19 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95102107, mouse embryo, Not specified

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

mouse embryo

description

Mouse teratocarcinoma

packaging

tube of 5 μg 95102107-DNA-5UG
pkg of vial of cells 95102107-1VL

growth mode

Adherent

karyotype

Euploid

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Differentiation studies

Biochem/physiol Actions

Mouse teratocarcinoma
The P19 cell line was derived from an embryonal carcinoma induced in a C3H/He strain mouse. The cells can be cloned at high efficiency in medium containing 0.1mM β-mercaptoethanol. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells in the presence of retinoic acid. Aggregates of P19 cells differentiate into cardiac and skeletal muscle in the presence of dimethyl sulfoxide (DMSO). In the presence of both retinoic acid and DMSO cells develop as if exposed to retinoic acid only.

Preparation Note

α MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2.5% Foetal Bovine Serum (FBS) + 7.5% Calf Serum (CS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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