SSE-5

Isolation of viruses from clinical samples, virus propagation

Fish Sockeye Salmon embryo

SSE-5 was initiated in 1964 from pooled embryos of Sockeye Salmon (Oncorynchus nerka). The cell line that is also known as Sockeye Salmon embryo-5, is susceptible to infectious pancreatic necrosis virus (IPNV), viral hemorrhagic septicaemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). For replication of salmonid viruses SSE-5 cells should be grown at 18°C.

MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 15-21°C; split every 10-14 days. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at leas

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.