J.CaM1.6

Study of apoptosis, signal transduction receptor analysis.

Human T cell, mutant derivative of Jurkat

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 10,11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18,19

The T cell leukaemia line J.CaM1.6 was derived from the parent cell line Jurkat E.6-1 (Sigma Catalogue number. 88042803). Jurkat cells were mutagenised with ethyl methanesulfonate and subsequently grown in phytohemagglutinin containing media for 2 weeks. J.CaM1.6 express a structurally normal but functionally defective CD3/Ti complex. The cells fail to exhibit inositolphospholipid metabolism or Ca²⁺ mobilisation in response to anti-CD3 or anti-Ti monoclonal antibodies. They display resistance to anti-CD3 induced apoptosis, constitutively express Fas and are sensitive to anti-Fas induced apoptosis. It was shown that J.CaM1.6 cells are defective in early signalling events that follow TCR stimulation owing to loss of lck tyrosine kinase function due to the lack of exon 7 in the lck transcript.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

To keep the cells in exponential growth, maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. T

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.