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tsa201 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96121229, human kidney, Epithelial-like

Synonym(s):

tsA-201 Cells

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About This Item

UNSPSC Code:
41106514
Biological source:
human kidney
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial-like

Key Documents

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biological source

human kidney

packaging

tube of 5 μg 96121229-DNA-5UG, pkg of vial of cells 96121229-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

tsa201 human cell line has been used for protein-binding studies, whole-cell patch clamp recordings and transfection studies.

Biochem/physiol Actions

Human embryonal kidney, SV40 transformed
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19
tsA201 is a transformed human kidney (293, Sigma catalogue no. 85120602) cell line stably expressing an SV40 temperature-sensitive T antigen. The cell line has been used in a variety of functional expression assays and has been reported to produce high levels of recombinant proteins. Transfection efficiency decreases over a period of several months, therefore it is advisable to lay down a frozen stock as a reserve. The cells have been eradicated from mycoplasma at ECACC. The identity of tsA201 and 293 has been confirmed by STR profiling.

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:8; cells are dislodged by mechanical agitation or EDTA solution; 5% CO2; 37°C. Do not let cells become over confluent as this can affect morphology.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

常规特殊物品
低风险生物材料
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