tsa201 Cell Line human

tsa201 human cell line has been used for protein-binding studies, whole-cell patch clamp recordings and transfection studies.

Human embryonal kidney, SV40 transformed

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

tsA201 is a transformed human kidney (293, Sigma catalogue no. 85120602) cell line stably expressing an SV40 temperature-sensitive T antigen. The cell line has been used in a variety of functional expression assays and has been reported to produce high levels of recombinant proteins. Transfection efficiency decreases over a period of several months, therefore it is advisable to lay down a frozen stock as a reserve. The cells have been eradicated from mycoplasma at ECACC. The identity of tsA201 and 293 has been confirmed by STR profiling.

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:8; cells are dislodged by mechanical agitation or EDTA solution; 5% CO₂; 37°C. Do not let cells become over confluent as this can affect morphology.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.