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About This Item
UNSPSC Code:
41106514
Biological source:
human unknown/unspecified
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
Not specified
Morphology:
Lymphoblastoid
biological source
human unknown/unspecified
packaging
tube of 5 μg 98052918-DNA-5UG, pkg of vial of cells 98052918-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
IL-5
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Leukemia studies, oncogene expression, differentiation assays
Biochem/physiol Actions
Human caucasian promyelocytic leukaemia
The clone 15 HL-60 has been derived from HL-60 cells (Sigma Catalogue number. 98070106) that had been cultured at an elevated pH of 7.6 - 7.8 for 2 months. Clone 15 HL-60 was picked from soft agar being selected for the ability to differentiate to eosinophils after treatment with butyric acid. Although 80% of the cells will exhibit this differentiation pattern initially, reversion will occur during culture leading to a higher percentage reverting to neutrophilic differentiation. Expression of IL-5 receptors and eosinophil granule proteins has been found (eosinophil major basic protein (MBP), eosinophil peroxidase (EPO), eosinophilotactions protein (ECP) and eosinophil derived neurotoxin (EDN). In addition, chemotaxis to eosinophil specific eosinophilotactius has been described.
Preparation Note
Maintain cultures between 3-9 x 100,000 cells per ml; 5% CO2; 37°C. Immediately after resuscitation use medium with 20% foetal bovine serum (FBS) to aid establishment of growing culture. Reduce FBS concentration to 10% once cells are well established in
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
常规特殊物品
低风险生物材料
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