LB316

Toxicity studies, hprt mutation analysis, DNA repair deficiencies

Established by EBV transformation of mononuclear cells from the peripheral blood of a 1 year old male. The patient was diagnosed with Xeroderma pigmentosum, complementation group D, with some features of Cockayne syndrome. Fibroblasts derived from the same individual are designated XP8BR.

Human lymphoblastoid, Xeroderma pigmentosum, EBV-transformed

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂; 37°C. Cells form floating aggregates.

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.