biological source
human blood
packaging
tube of 5 μg 98062336-DNA-5UG, pkg of vial of cells 98062336-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Toxicity studies, hprt mutation analysis, DNA repair deficiencies
Biochem/physiol Actions
Established by EBV transformation of mononuclear cells from peripheral blood of a 41 year old male. The patient was shown to be heterozygote for Xeroderma pigmentosum (XP) group A, a rare, autosomal recessive disease with DNA repair deficiencies. A cell line established from a child of this patient with XP is available (LB708, Sigma Catalogue number. 98062337).
Human lymphoblastoid, Xeroderma pigmentosum heterozygote, EBV-transformed
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C.
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.