CTX TNA2

Studies of the development and biochemistry of the central nervous system.

Established by transfecting cultures of primary astrocytes from brain frontal cortex tissue of 1 day old sprague-dawley rats with a DNA construct containing the oncogenic early region of SV40. Transcriptional control was effected using the human GFAP promoter (pGFA-SV-TE) and the murine phosphoglycerate kinase promoter (pPGK-neo). Cloning was achieved using G418. The cells are phenotypically similar to type 1 astrocytes. CTX TNA2 displays 20% positive staining for glial fibrillary acid protein (GFAP) and a β alanine inhibitable high affinity uptake mechanism for gamma amino butyric acid (GABA). α-2-macroglobulin production is similar to that found in primary astrocytes, but transferrin production is reduced. The cell line has been shown not to produce proenkephalin A, galactoceebroside or to express the 04 or A2B5 epitopes characteristic of type 2 astrocytes. Immunostaining has shown the SV40 T antigen to be present in over 95% of cells.

Rat Astrocyte, Transfected

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Subculture before flasks become confluent or cells will detach in sheets reducing the effectiveness of trypsin

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.