biological source
human lung
packaging
tube of 5 μg 98110201-DNA-5UG, pkg of vial of cells 98110201-1VL
growth mode
Adherent/aggregates in suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
SCLC studies, cytotoxicity assays, immunological studies
Biochem/physiol Actions
Human Caucasian lung small cell carcinoma, large cell variant
SHP-77 was established in 1977 being derived from a non-encapsulated primary lung tumor from the apical portion of the upper lobe of the left lung. The patient was a 54 year old male with blood type O, Rh+. The cell line has a variety of unusual characteristics including the morphology of an undifferentiated large cell variant of small cell lung carcinoma (SCLC) but biochemical properties of a classical SCLC. SHP-77 express neural cell adhesion molecule (NCAM), NHK-1, CD56 and the cell surface leucocyte antigen Leu-7(HNK-1;CD57). Other neuroendocrine markers are L-dopa decarboxylase and dense core secretory granules. Gland formation and intracytoplasmic lamellar bodies have been shown by electron microscopy. This cell line is useful as in vitro target in 51Cr and III In release cytotoxicity assays. They are suitable for in vitro nude mice assays for evaluation of immune reactivity of cells and serum from lung cancer patients. In addition, they serve to evaluate the immune status of patients with SCLC who received radiation or chemotherapy treatment. SHP-77 cells are tumorigenic in nude mice and grow as circumscribed nodules without metastases formation. They have a reported doubling time of 96 hours.
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8
D16S539: 11
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 9,11
vWA: 16
CSF1PO: 10,11
D13S317: 8
D16S539: 11
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 9,11
vWA: 16
Preparation Note
Maintain cultures between 4-9 x 100,000 cells / ml; 5% CO2; 37°C. Cells grow as mixture of loosely adherent and floating clusters.
RPMI 1640 + 2mM Glutamine + 10% Heat Inactivated Foetal Bovine Serum (HI-FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.