biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 99061826-DNA-5UG, pkg of vial of cells 99061826-1VL
ImmunoDon
(C57BL/6 x BALB/c)F1
Myeloma
P3x63Ag8.653
Reactivity
Anti-HLA DR7, DRw10,DRw53
technique(s)
cell culture | mammalian: suitable
isotype
IgG3
shipped in
dry ice
storage temp.
−196°C
Immunogen
B cell line Leakar HLA A 2,24; B13,35;C4; DR 7,3
Application
Immunofluorescence, cytotoxicity
Biochem/physiol Actions
LY9 was produced from a (C57BL/6 x BALB/c)F1 mouse immunised with a single i.p. injection of the lymphoblastoid B-cell line Leakar and B. pertussis adjuvant. The genotype of the B-cell donor for this line was HLA-A2,B13, Cw- DR7, DQ 2/A24,B13, Cw-,DR7 B35, Cw4, DR3, DQ2. Mice spleen was removed 3 days later and fused with the myeloma P3x63Ag8.653. LY9 monoclonal antibodies were shown to react with cells expressing DRw53, DR7 (in the absence of DRw53) and DRw10. LY9 is also known as LK261.
Mouse x Mouse hybridoma
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 1 mM NaP + 10% FBS
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.