biological source
eel liver
packaging
tube of 5 μg 99072812-DNA-5UG, pkg of vial of cells 99072812-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Eel liver
The hepatocyte cell line Hepa-E1 was derived from water eel. The cells show no contact inhibition and have been reported to show apoptosis in the presence of actinomycin D, humic acid or TGF-β 1. Normal culture conditions are at 26-28°C, but maintenance at 4°C for up to 400 days was described as well.
Preparation Note
E-RDF (Kyokuto Pharmaceuticals)+ 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% trypsin or trypsin/EDTA 26-28°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.