General description
RDO is a very rapid bone decalcifier. The hydrochloric acid component of RDO reacts with calcium in mineralized tissues to form soluble calcium salts.
Application
1. To insure adequate fixation and decalcification, specimens should be trimmed to less than 1 cm thickness.
2. Most specimens can be decalcified in 4 hours or less.
3. Use adequate volume of RDO to tissue; a 20:1 volume ration of RDO to tissue is recommended.
4. The key determinations for time required for decalcification are size and density of the specimen. Most mature bones of 1 cm size are decalcified in 4-6 hours; smaller spongy bone in 2-4 hours. Bone biopsies are decalcified in 30-60 minutes. Teeth and entire femur heads may require overnight treatment.
5. If RDO action is too rapid, dilute with distilled or deionized water. Good results have been obtained on bone marrow biopsies with a 3:1 dilution (RDO to water).
6. Determine the point of decalcification using standard methods (i.e. X-ray, flexibility, chemical analysis).
7. DO NOT OVER DECALCIFY. RDO action is very rapid. Do not leave bone specimen in RDO for several days.
8. FORMALIN FIXATION AND RDO DECALCIFICATION SHOULD NOT BE COMBINED. Hydrochloric acid and formaldehyde vapors have been reported to form a potent carcinogen. Prior fixation with formalin is permissible.
2. Most specimens can be decalcified in 4 hours or less.
3. Use adequate volume of RDO to tissue; a 20:1 volume ration of RDO to tissue is recommended.
4. The key determinations for time required for decalcification are size and density of the specimen. Most mature bones of 1 cm size are decalcified in 4-6 hours; smaller spongy bone in 2-4 hours. Bone biopsies are decalcified in 30-60 minutes. Teeth and entire femur heads may require overnight treatment.
5. If RDO action is too rapid, dilute with distilled or deionized water. Good results have been obtained on bone marrow biopsies with a 3:1 dilution (RDO to water).
6. Determine the point of decalcification using standard methods (i.e. X-ray, flexibility, chemical analysis).
7. DO NOT OVER DECALCIFY. RDO action is very rapid. Do not leave bone specimen in RDO for several days.
8. FORMALIN FIXATION AND RDO DECALCIFICATION SHOULD NOT BE COMBINED. Hydrochloric acid and formaldehyde vapors have been reported to form a potent carcinogen. Prior fixation with formalin is permissible.
Legal Information
Accumate is a trademark of Sigma-Aldrich Co. LLC
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Danger
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Skin Corr. 1A
存储类别
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable