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Merck
CN

GE11-0034-00

His Buffer Kit

Cytiva 11-0034-00

Synonym(s):

His Buffer

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About This Item

UNSPSC Code:
41115700
NACRES:
NA.25

Key Documents

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Technical Service
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sterility

non-sterile

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 11-0034-00

Related Categories

General description

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, method for optimization of imidazole concentration in the binding buffer is described.

Features and Benefits

His Buffer Kit can be used in combination with a number of column types, such as His SpinTrap, His MultiTrap™ FF, His MultiTrap™ HP, His GraviTrap, HisTrap™ FF, HisTrap™ FF crude and HisTrap™ HP

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HisTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H314

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Regulatory Information

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Articles

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Protocols

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Gravity-flow Purification using His GraviTrap™ and His GraviTrap™ Kit

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.

Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni

This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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