GE17-0042-02
Sephadex® G-50
Fine, Cytiva, pack of 500 g
Synonym(s):
SEC resin, buffer exchange resin, chromatography resin, cross-linked dextran, cytiva 17-0042-02, desalting matrix, filtration gel, size-exclusion resin
form
resin
crosslinking
cross-linked
mol wt
protein Mr 3-20 kDa (fractionation range)
protein Mr 30 kDa (exclusion limit)
feature
autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging
pack of 500 g
manufacturer/tradename
Cytiva
parameter
<Darcy´s Law flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
cross-linked dextran
matrix active group
dextran
swelling
1 g swells to 13-16 mL
particle size
20-80 μm (dry)
41-171 (wet)
cleaning in place
2-13
working range
2-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
SMILES string
O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
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General description
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for purification of DNA from small molecules by gel filtration
- Small bead size for shorter diffusion distances
- Classic gel filtration medium.
Preparation Note
Analysis Note
Legal Information
Storage Class Code
13 - Non Combustible Solids
Regulatory Information
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