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Merck
CN

GE17-0402-03

HiTrap® Protein A High Performance

Cytiva 17-0402-03, pack of 2 × 1 mL

Synonym(s):

affinity column, chromatography column, mAb purification column, prepacked column, protein A column

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

Key Documents

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form

prepacked column

crosslinking

cross-linked

packaging

pack of 2 × 1 mL

manufacturer/tradename

Cytiva 17-0402-03

storage condition

(20% Ethanol)

parameter

0.5 Mpa max. pressure
1 mL/min flow rate
4 mL/min max. flow rate

technique(s)

liquid chromatography (LC): suitable

bed volume

1 mL

column I.D. × L

7 mm × 25 mm

size

matrix

highly cross-linked agarose, spherical

matrix active group

Protein A

avg. part. size

34 μm

cleaning

2-10

working range

3-9

capacity

~20 mg binding capacity (human IgG / mL resin)

separation technique

affinity

storage temp.

2-8°C

Related Categories

General description

HiTrap® protein A HP is a prepacked protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.

Features and Benefits

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as ÄKTA® design or FPLC™ System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

Protocols

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

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