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About This Item
ligand
iminodiacetic acid
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-0409-03
parameter
<20 mL/min flow rate, 42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
size
avg. part. size
34 μm
cleaning
2-14
working range
3-13
General description
HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTAdesign or FPLC™ Systems.
Features and Benefits
- For optimizing purification of histidine-tagged proteins using different kinds of metal ions.
- Conveniently charge with your metal of choice.
- Iminidoacetic acid coupled to Sepharose™ High Performance.
- Purifying proteins with exposed histidine groups via metal ion complex formation.
- Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System.
- Packed with Chelating Sepharose™ High Performance
- Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System
Preparation Note
Analysis Note
Other Notes
Legal Information
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signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Protocols
Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Articles
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GE17-0409-03 | 04061837620188 |