ligand
Cibacron Blue F3G-A
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-0412-01
parameter
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
size
avg. part. size
34 μm
cleaning
3-13
working range
4-12
General description
HiTrap® Blue HP is prepacked with Blue Sepharose™ High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins.
Application
Blue Sepharose™ High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some Proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.
Features and Benefits
- Can be used for depletion of albumin from serum.
- Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked Agarose medium and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning.
- The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids