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GE17-0413-01

HiTrap® Blue High Performance

Cytiva 17-0413-01, pack of 1 × 5 mL

Synonym(s):

Cibacron Blue F3G-A column, albumin affinity column, chromatography column, interferon affinity column, prepacked column

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151816
Technical Service
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ligand

Cibacron Blue F3G-A

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 17-0413-01

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

size

avg. part. size

34 μm

cleaning

3-13

working range

4-12

General description

HiTrap® Blue HP is prepacked with Blue Sepharose™ High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins.

Application

Blue Sepharose™ High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some Proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.

Features and Benefits

  • Can be used for depletion of albumin from serum.
  • Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked Agarose medium and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning.
  • The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

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pictograms

Flame
GHS02

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Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Protocols

Purification of NAD+ and ATP-dependent Kinases

Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.

Purification or Removal of Albumin

This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.

Performing a Separation or Removal of Albumin with HiTrap® Blue HP and Blue Sepharose 6 Fast Flow

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

View All Protocols

Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Global Trade Item Number

SKUGTIN
GE17-0413-0104061837620218