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Merck
CN

GE17-0440-03

Con-A Sepharose™ 4B

Cytiva 17-0440-03, pack of 5 mL

Synonym(s):

chromatography resin, concanavalin A resin, gel, glycolipids affinity resin, glycoprotein affinity resin, matrix

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

Key Documents

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Technical Service
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ligand

concanavalin A (con A)

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-0440-03

storage condition

(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-9

working range

4-6

storage temp.

2-8°C

Related Categories

General description

Con A Sepharose™ 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

Application

Con A Sepharose™ is Concanavalin A coupled to Sepharose™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoProteins, polysaccharides and glycolipids

Features and Benefits

  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Group-specific adsorbent for molecules containing sugars.
  • Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
  • Con A coupled to Sepharose™ 4B via cyanogen bromide activation

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Protocols

Purification or Removal of Glycoproteins and Polysaccharides

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

用于亲和层析的色谱柱填充和制备实验方案

该页面展示了如何使用Cytiva的Tricorn™或XK色谱柱进行亲和层析色谱柱填充和制备。

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