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GE17-0444-01

Lentil Lectin-Sepharose™ 4B 25 mL

Cytiva 17-0444-01

Synonym(s):

Sepharose 4B

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500
Technical Service
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ligand

lentil lectin

packaging

pkg of 25 mL

manufacturer/tradename

Cytiva 17-0444-01

storage condition

(20% Ethanol, PBS pH 7)

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

3-12

working range

3-10

storage temp.

2-8°C

General description

Lentil Lectin Sepharose™ media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for α-D-mannose and α-D-glucose compared to Con A.

Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose™ 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.

Features and Benefits

  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues
  • Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl
  • Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
  • Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Protocols

Purification or Removal of Glycoproteins and Polysaccharides

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

View All Protocols

Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Global Trade Item Number

SKUGTIN
GE17-0444-0104061837620249