ligand
calmodulin
packaging
pack of 10 mL
manufacturer/tradename
Cytiva 17-0529-01
storage condition
(20% Ethanol)
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
4-9
working range
4-9
storage temp.
2-8°C
General description
Calmodulin Sepharose™ 4B affinity media for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.
Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca2+ complexes are particularly stable.
Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca2+ complexes are particularly stable.
Features and Benefits
- For single-step purification of native calmodulin-binding proteins
- Suitable for tandem affinity purification (TAP) of protein complexes
- Purification of calmodulin-regulated proteins from all eukaryotic cells
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
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Warning
hcodes
存储类别
3 - Flammable liquids