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Merck
CN

GE17-0855-02

NICK-Column 50 ST

Cytiva 17-0855-02

Synonym(s):

17-0855-02, DNA column, G-50, NICK columns, Sephadex, columns, filters, nick-translated DNA clolumn

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52
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packaging

pkg of 50 columns

manufacturer/tradename

Cytiva 17-0855-02

bed size

9 mm × 20 mm

column I.D.

9 mm

particle size

50-150 μm (dry)

capacity

100 μg binding capacity

General description

NICK Columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labeled probe from unincorporated labeled nucleotides.

NICK Columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labeled probe from unincorporated labeled nucleotides. They are ready for immediate use, and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered. Maximum sample volume is 100 μ.

Features and Benefits

  • For rapid purification of labeled DNA (20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography.
  • Prepacked with Sephadex® G-50 DNA Grade (see illustra Sephadex® G-50 and G-100 DNA Grade) in distilled water with 0.15% Kathon CG/ICP Biocide.
  • Can accommodate sample volumes up to 100 μ.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephadex is a registered trademark of Cytiva
illustra is a trademark of Cytiva

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Hazard Statements

Storage Class Code

12 - Non Combustible Liquids

Regulatory Information

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