GE17-1154-01
HiTrap® Q High Performance
Cytiva 17-1154-01, pack of 5 × 5 mL
Synonym(s):
anion exchange columns, Q Sepharose High Performance columns
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About This Item
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 10 MDa (exclusion limit)
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-1154-01
storage condition
(20% ethanol)
parameter
20 mL/min max. flow rate
42 psi
5 mL/min flow rate (recommended)
technique(s)
liquid chromatography (LC): suitable
bed volume
5 mL
column I.D. × L
16 mm × 25 mm
matrix
cross-linked agarose, spherical
matrix active group
-CH2N+(CH3)3, quaternary ammonium
particle size
24-44 μm
avg. part. size
34 μm
cleaning
2-14
working range
2-12
capacity
~ 70 mg BSA/mL resin binding capacity
0.14-0.2 mmol Cl-/mL ion exchange capacity
separation technique
anion exchange
shipped in
ambient
storage temp.
2-30°C
Related Categories
General description
Application
Features and Benefits
- 34 μm bead size for high-performance, high-resolution purifications.
- Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
- Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
- Strong quaternary ammonium (Q) anion exchanger
Preparation Note
Analysis Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
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Articles
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protocols
Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
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