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GE17-1154-01

HiTrap® Q High Performance

Cytiva 17-1154-01, pack of 5 × 5 mL

Synonym(s):

anion exchange columns, Q Sepharose High Performance columns

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151816
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form

prepacked column

crosslinking

cross-linked

mol wt

protein Mr 10 MDa (exclusion limit)

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-1154-01

storage condition

(20% ethanol)

parameter

20 mL/min max. flow rate, 42 psi, 5 mL/min flow rate (recommended)

technique(s)

liquid chromatography (LC): suitable

bed volume

5 mL

column I.D. × L

16 mm × 25 mm

matrix

cross-linked agarose, spherical

matrix active group

-CH2N+(CH3)3, quaternary ammonium

particle size

24-44 μm

avg. part. size

34 μm

cleaning

2-14

working range

2-12

capacity

~ 70 mg BSA/mL resin binding capacity, 0.14-0.2 mmol Cl-/mL ion exchange capacity

separation technique

anion exchange

shipped in

ambient

storage temp.

2-30°C

General description

HiTrap® Q HP are prepacked, ready-to-use Q Sepharose™ High Performance strong anion exchange columns for high-resolution, small-scale protein purification.

Application

Techniques & Applications: Separation of protein using a strong anion exchange medium prepacked in the HiTrap® column format.

Features and Benefits

  • 34 μm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
  • Strong quaternary ammonium (Q) anion exchanger

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

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pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Protocols

Refolding: Ion Exchange Chromatography

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

View All Protocols

Articles

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

View All Articles

Global Trade Item Number

SKUGTIN
GE17-1154-0104061837621154