material
polypropylene
mol wt
10000-100000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 17-1165-01
parameter
<1.0 mL/min flow rate, <5 mL sample volume, 0.15 Mpa (22 psi)
bed volume
120 mL
column L × I.D.
60 cm × 16 mm
matrix
dextran-acrylamide particle platform, allyl dextran and N, N’-methylenebisacrylamide, polymeric
matrix active group
dextran-acrylamide phase
avg. part. size
50 μm
cleaning
2-13
working range
3-11
separation technique
size exclusion (SEC)
General description
HiPrep™ 16/60 Sephacryl® S-100 HR are prepacked gel filtration columns for preparative separation of biomolecules of up to 5 mL sample volume. Sephacryl® S-100 is used for separating peptides and small proteins.
Application
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 5 mL sample volume.
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Features and Benefits
- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
- Gel filtration columns for low to medium pressure AKTA systems.
- Well-established in industrial production.
- The HiPrep™ Sephacryl® series offers reproducible separations over a wide molecular weight range.
- Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids