form
prepacked column
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 17-1178-01
storage condition
(0.2 M sodium acetate in 20% ethanol)
parameter
1-10 mL/min flow rate, 1.5 Mpa max. pressure
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
6.4 mm × 30 mm
matrix
polystyrene/divinylbenzene
matrix active group
sulfonate group (–O–CH2–CHOH–CH2–O–CH2–CHOH–CH2–SO3-)
particle size
~15 μm
cleaning
1-14
operating pH
2-13
capacity
80 mg binding capacity (lysozyme / mL resin)
separation technique
cation exchange
storage temp.
2-30°C
General description
RESOURCE™ S columns are prepacked with SOURCE™ 15S, a strong cation exchanger for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Features and Benefits
- SOURCE™ 15Q and 15S media are well suited for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
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Warning
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存储类别
3 - Flammable liquids