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Merck
CN

GE17-1279-03

rProtein A Sepharose™ Fast Flow

Cytiva 17-1279-03, pack of 200 mL

Synonym(s):

antibody affinity medium, antibody purification resin

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500

Key Documents

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form

resin

crosslinking

cross-linked

packaging

pack of 200 mL

manufacturer/tradename

Cytiva 17-1279-03

storage condition

(20% Ethanol)

technique(s)

liquid chromatography (LC): suitable

matrix

4% cross-linked agarose

matrix active group

Recombinant protein A (E. coli)

particle size

~90 μm

cleaning in place

3-12

optimum pH

3-10

capacity

~35 mg binding capacity (human lgG / ml medium)

separation technique

affinity

storage temp.

2-8°C

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Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Maida Romera-Branchat et al.
Cell reports, 31(9), 107717-107717 (2020-06-04)
Flowering of many plant species depends on interactions between basic leucine zipper (bZIP) transcription factors and systemically transported florigen proteins. Members of the genus Arabidopsis contain two of these bZIPs, FD and FDP, which we show have largely complementary expression
Lawrence T Wang et al.
Cell reports, 38(7), 110367-110367 (2022-02-17)
L9 is a potent human monoclonal antibody (mAb) that preferentially binds two adjacent NVDP minor repeats and cross-reacts with NANP major repeats of the Plasmodium falciparum circumsporozoite protein (PfCSP) on malaria-infective sporozoites. Understanding this mAb's ontogeny and mechanisms of binding
Lawrence T Wang et al.
Immunity, 53(4), 733-744 (2020-09-19)
Discovering potent human monoclonal antibodies (mAbs) targeting the Plasmodium falciparum circumsporozoite protein (PfCSP) on sporozoites (SPZ) and elucidating their mechanisms of neutralization will facilitate translation for passive prophylaxis and aid next-generation vaccine development. Here, we isolated a neutralizing human mAb

Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

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Protocols

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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