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About This Item
NACRES:
NA.56
UNSPSC Code:
23151816
ligand
phenyl
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-1353-01
parameter
<4 mL/min flow rate, 42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
3-13
suitability
suitable for bioprocess medium
General description
HiTrap® Phenyl FF (Low Sub) are prepacked Phenyl Sepharose™ 6 Fast FLow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.
Features and Benefits
- Provide convenient and fast small-scale Protein purifications.
- Phenyl Sepharose™ 6 Fast FLow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring Low to medium hydrophobicity. Two levels of substitution on Phenyl Sepharose™ 6 Fast FLow help find optimal selectivity and binding capacity.
- Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA® design.
- High flow rates and high capacities.
- Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
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signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Protocols
This page discusses various aspects of sample preparation for chromatographic purification.
Articles
Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GE17-1353-01 | 04061837621390 |