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About This Item
ligand
quaternary amine
description
Ion Exchanger Type (value)
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5053-01
parameter
<4 mL/min flow rate, 42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
2-12
suitability
suitable for bioprocess medium
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General description
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
Features and Benefits
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High flow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
Preparation Note
Analysis Note
Other Notes
Legal Information
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Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Articles
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page shows how to perform a purification of His-tagged membrane proteins.
Protocols
Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
This page shows how to prepare samples for purification with affinity chromatography.
This page covers the use of Sepharose Fast Flow for purification of proteins.
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