ligand
sulphopropyl
description
Ion Exchanger Type (value)
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5054-01
parameter
flow rate, 42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
3-14
working range
4-13
suitability
suitable for bioprocess medium
General description
HiTrap® SP FF is prepacked with SP Sepharose™ Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
Application
Q, SP, DEAE, and CM Sepharose™ Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.
Features and Benefits
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High fLow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids