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GE17-5087-02

HiPrep™ 26/10 Desalting

20-80 μm particle size (dry), bed H × diam. 10 cm × 2.6 cm, pack of 4 ea

Synonym(s):

HiPrep Desalting Column, Desalting Column

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About This Item

NACRES:
SB.52
UNSPSC Code:
41115700
Technical Service
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form

prepacked column

crosslinking

cross-linked

mol wt

protein Mr 5 kDa (exclusion limit)

packaging

pack of 4 ea

manufacturer/tradename

Cytiva 17-5087-02

storage condition

(20% ethanol)

parameter

≤15 mL sample volume, 5 bar max. pressure, 9-31 mL/min flow rate

technique(s)

liquid chromatography (LC): suitable

bed H × diam.

10 cm × 2.6 cm

bed volume

53 mL

matrix

cross-linked dextran

matrix active group

dextran-epichlorohydrin phase, dextran

particle size

20-80 μm (dry)

cleaning

2-13

working range

2-13

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

General description

HiPrep™ 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Application

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps

Features and Benefits

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

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pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Protocols

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Automated Antibody Purification with ÄKTA Systems

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

View All Articles

Global Trade Item Number

SKUGTIN
GE17-5087-0204061838551603